Generator

Part:BBa_K624061:Design

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

pYMB essentials + RBS(trunc.) + LLO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2558
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

LLO: PCR amplification from the genomic DNA of Listeria monocytogenes

References

pYMB:

Y. Okamura, et al. Design and Application of a new cryptic plasmid-based shuttle vector for Magnetospirillum magneticum. Appl. Environ. Microbiol., 69 (2003), pp. 4274–4277.